It is requested to please suggest that i have sầu a standard method that gives answer in MPN/g và the requirement is that the result of Food microbiology must be reported in CFU/g. Kindly mention any personal work or research.

MPN is most probable number between 25-300 count of microbes. if the MPN/g less than 25 means zero CFU/g

There is no way to lớn convert CFU khổng lồ MPN and vice versa. CFU's are the Colony Forming Units in a given sample volume as in, these ARE the colony forming units in 100mLs of a sample. CFU's are definitive. MPN's are the Most Probable Number of cfu's in a given sample volume as in, there are PROBABLY this many cfu's in 100 mLs of a sample. MPN is an estimate of probability. (answered by Wiki)

actually, the term describes the method, if you use agar then the growth is described as CFU. if you use a liquid medium, the growth is described as MPN. and again, it's not a mistake to lớn use the terms interchangeably.

" The MPN should be judged as an estimate of growth units (GUs) or colony-forming units (**CFUs**) instead of individual bacteria. For simplicity, however, this appendix will speak of these GUs or CFUs as individual bacteria."

The simple answer is that they are equivalent- one MPN is equal to one CFU. Both units measure the estimated number of bacteria in a water sample. Both are recognized by a variety of scientific & regulatory bodies worldwide including the US Environmental Protection Agency (EPA) and the International Standardization Organization (ISO). The difference is in how the measurement is obtained. The use of either MPN or CFU is based on the method used for the detection of bacteria and both are valid measurements for bacteria limits.

For CFUs, bacteria grow on a solid medium, like agar. Afterward, colonies of bacteria are counted. For an MPN measurement, samples grown in a liquid medium, lượt thích multiple tube fermentation & Colilert. Positive sầu wells/tubes are then counted and an MPN conversion table is used lớn generate a numeric result.

Both measurements are well-established means lớn estimate the number of bacteria in a sample, and both carry a 95% confidence interval.

I appreciate your quite elaborate answer and agree too on the same except the fact that you have sầu probably assumed that CFU/g & MPN are estimates of bacteriological unique parameters of water alone.

Further it is also true that for both water, milk & few other dairy products this is true và valid as this generally applies lớn fluid products as listed above.

However, when it comes to variety of solid food stuffs it is CFU/g that has been prescribed by regulatory authorities world over rather than MPN protocol.

Further MPN cannot directly be equated to CFU/g or CFU/ml though one may say that both are somewhat equivalent measures of bacteriological chất lượng parameters & I have already dwelt in more details earlier on the same thread lớn highlight the difference between two.

There is one correction* in the sense that I had posted my response earlier but not on this thread. But I distinctly remember a similar question came up for discussion on RG platform some time baông xã.

MPN is a serial dilution tests that is used khổng lồ estimate the concentration of viable organism in a sample. This method is useful with samples that contain particulate material that interferes with plate count enumeration methods. Both the MPN và plating method (CFU) are equivalent.

MPN = samples grown in a liquid medium (MPN) & the positive tubes are then counted và an MPN conversion table is used to lớn generate a numeric result.

Both measurements are well-established means to estimate the number of bacteria in a sample, and both carry a 95% confidence interval.

I have sầu tested my sample for coliform analysis using colony counting method and obtained a result of 1 X 102 cfu/g. However, I am comparing with a standard of MPN/g. Hence, I have to lớn convert that cfu/g inkhổng lồ MPN/g. Is there any way that I can vày that?

I want khổng lồ calculate the colony forming unit of a bacterium which is frozen in glycerol solution. I will thử nghiệm an antibacterial surface so I have sầu lớn know how many bacteria there are in the LB medium before putting them onkhổng lồ the surface. Is there a way khổng lồ calculate it?

I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have sầu one study which reports its microbial values in MPN. What should I do?

As far as I know, dilution factor = volume of sample/total volume and CFU = (no colony x dilution factor)/volume plated in mL. For example, if I added 10gr of sample inlớn 90mL sterilized Ringer's solution. Is it 10-1? After that, i made serial dilutions, & transfer 1mL from 10-1 lớn second tube (9mL as well). Then this will be10-2? Then, for example, I take 1cc from the first tube (not from the diluted primary sample) and overlay it on a petri with a medium và the final colonies are 25. The right logcfu/mL is (25*1)/0.1?

i work on powder infant food , i take 1g then i put it in 70ml liquid media in flask & incubate it for 24h , then i take 1ml và diluted it three times in 9ml of water after that i take 0.2ml from tube 2 (1:100) then i spread it on solid media in petri dish , incubate for 24h và the count of colonies was 80, i calculate cfu & the result 80×100 CFU/ml is that correct? i need cfu/g because the orginal sample powder food? Can you help me?

as Changing the numbers lớn the logarithm makes it easier lớn see small numbers and large numbers on the same graph.

For my case, I have sầu calculated the CFU/g for fungi using selective agar, so I vì chưng not get much fungi in which plate (10-50 per colonies in each plate).

for example, the highest CFU/g i get is 5000 cfu/g only (the value is not very big), so is it necessary lớn convert it to lớn log value? especially for analyzing the data using ANOVA?5 g of soil was placed into 10 ml water. The solution was serial diluted 10x (1/10). then 0.1 ml of the dilution was spread onkhổng lồ the agar plate.

Can anyone suggest me the calculation for converting MPN Index/100 ml lớn MPN/100 ml? In general we kiểm tra the positive tubes and verify with the Interpretation chart as per APHA. But how khổng lồ calculate MPN/100 ml based on 5 tube method và how many dilutions are to lớn be followed? Please.

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I appreciate you for the most tricky technical question that has been posted on RG so far và we all are awaiting for the satisfactory answer yet.

Both methodologies are based on certain presumptions before the statistical analysis is applied khổng lồ arrive sầu at the conclusion/correct answer.

Despite the fact that Mathematics and Statistics are considered as mother of all sciences và it is true indeed still there is a scope to believe sầu that there could be some complications of statistical methods which are rather beyond comprehension.

There is no point in saying that "the mean human body toàn thân temperature is 37 degree Celcius và everything is fine when in an imaginary situation if you try to lớn visualize following situation":

The half of the body toàn thân is kept in a deep freeze at minus 200 degree Celcius and the other half is in the muffle furnace at 237 degree Celsius.

I appreciate your quite elaborate answer & agree too on the same except the fact that you have sầu probably assumed that CFU/g and MPN are estimates of bacteriological chất lượng parameters of water alone.

Further it is also true that for both water, milk và few other dairy products this is true và valid as this generally applies to fluid products as listed above.

However, when it comes to lớn variety of solid food stuffs it is CFU/g that has been prescribed by regulatory authorities world over rather than MPN protocol.

Further MPN cannot directly be equated to lớn CFU/g or CFU/ml though one may say that both are somewhat equivalent measures of bacteriological unique parameters và I have already dwelt in more details earlier on the same thread lớn highlight the difference between two.

MPN is most probable number between 25-300 count of microbes. if the MPN/g less than 25 means zero CFU/g

There is no way to lớn convert CFU khổng lồ MPN and vice versa. CFU's are the Colony Forming Units in a given sample volume as in, these ARE the colony forming units in 100mLs of a sample. CFU's are definitive. MPN's are the Most Probable Number of cfu's in a given sample volume as in, there are PROBABLY this many cfu's in 100 mLs of a sample. MPN is an estimate of probability. (answered by Wiki)

actually, the term describes the method, if you use agar then the growth is described as CFU. if you use a liquid medium, the growth is described as MPN. and again, it's not a mistake to lớn use the terms interchangeably.

" The MPN should be judged as an estimate of growth units (GUs) or colony-forming units (

The simple answer is that they are equivalent- one MPN is equal to one CFU. Both units measure the estimated number of bacteria in a water sample. Both are recognized by a variety of scientific & regulatory bodies worldwide including the US Environmental Protection Agency (EPA) and the International Standardization Organization (ISO). The difference is in how the measurement is obtained. The use of either MPN or CFU is based on the method used for the detection of bacteria and both are valid measurements for bacteria limits.

For CFUs, bacteria grow on a solid medium, like agar. Afterward, colonies of bacteria are counted. For an MPN measurement, samples grown in a liquid medium, lượt thích multiple tube fermentation & Colilert. Positive sầu wells/tubes are then counted and an MPN conversion table is used lớn generate a numeric result.

Both measurements are well-established means lớn estimate the number of bacteria in a sample, and both carry a 95% confidence interval.

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I appreciate your quite elaborate answer and agree too on the same except the fact that you have sầu probably assumed that CFU/g & MPN are estimates of bacteriological unique parameters of water alone.

Further it is also true that for both water, milk & few other dairy products this is true và valid as this generally applies lớn fluid products as listed above.

However, when it comes to variety of solid food stuffs it is CFU/g that has been prescribed by regulatory authorities world over rather than MPN protocol.

Further MPN cannot directly be equated to CFU/g or CFU/ml though one may say that both are somewhat equivalent measures of bacteriological chất lượng parameters & I have already dwelt in more details earlier on the same thread lớn highlight the difference between two.

There is one correction* in the sense that I had posted my response earlier but not on this thread. But I distinctly remember a similar question came up for discussion on RG platform some time baông xã.

MPN is a serial dilution tests that is used khổng lồ estimate the concentration of viable organism in a sample. This method is useful with samples that contain particulate material that interferes with plate count enumeration methods. Both the MPN và plating method (CFU) are equivalent.

MPN = samples grown in a liquid medium (MPN) & the positive tubes are then counted và an MPN conversion table is used to lớn generate a numeric result.

Both measurements are well-established means to estimate the number of bacteria in a sample, and both carry a 95% confidence interval.

I have sầu tested my sample for coliform analysis using colony counting method and obtained a result of 1 X 102 cfu/g. However, I am comparing with a standard of MPN/g. Hence, I have to lớn convert that cfu/g inkhổng lồ MPN/g. Is there any way that I can vày that?

I want khổng lồ calculate the colony forming unit of a bacterium which is frozen in glycerol solution. I will thử nghiệm an antibacterial surface so I have sầu lớn know how many bacteria there are in the LB medium before putting them onkhổng lồ the surface. Is there a way khổng lồ calculate it?

I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have sầu one study which reports its microbial values in MPN. What should I do?

As far as I know, dilution factor = volume of sample/total volume and CFU = (no colony x dilution factor)/volume plated in mL. For example, if I added 10gr of sample inlớn 90mL sterilized Ringer's solution. Is it 10-1? After that, i made serial dilutions, & transfer 1mL from 10-1 lớn second tube (9mL as well). Then this will be10-2? Then, for example, I take 1cc from the first tube (not from the diluted primary sample) and overlay it on a petri with a medium và the final colonies are 25. The right logcfu/mL is (25*1)/0.1?

i work on powder infant food , i take 1g then i put it in 70ml liquid media in flask & incubate it for 24h , then i take 1ml và diluted it three times in 9ml of water after that i take 0.2ml from tube 2 (1:100) then i spread it on solid media in petri dish , incubate for 24h và the count of colonies was 80, i calculate cfu & the result 80×100 CFU/ml is that correct? i need cfu/g because the orginal sample powder food? Can you help me?

as Changing the numbers lớn the logarithm makes it easier lớn see small numbers and large numbers on the same graph.

For my case, I have sầu calculated the CFU/g for fungi using selective agar, so I vì chưng not get much fungi in which plate (10-50 per colonies in each plate).

for example, the highest CFU/g i get is 5000 cfu/g only (the value is not very big), so is it necessary lớn convert it to lớn log value? especially for analyzing the data using ANOVA?

Can anyone suggest me the calculation for converting MPN Index/100 ml lớn MPN/100 ml? In general we kiểm tra the positive tubes and verify with the Interpretation chart as per APHA. But how khổng lồ calculate MPN/100 ml based on 5 tube method và how many dilutions are to lớn be followed? Please.